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FENS1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FENS1 Polyclonal Antibody AP55079
    Sample:
    Lane 1: U87MG (Human) Cell Lysate at 30 ug
    Lane 2: HepG2 (Human) Cell Lysate at 30 ug
    Lane 3: U2os (Human) Cell Lysate at 30 ug
    Lane 4: A431 (Human) Cell Lysate at 30 ug
    Primary:
    Anti-FENS1 (bs-13169R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 46 kD
    Observed band size: 46 kD
    detail
  • WB - FENS1 Polyclonal Antibody AP55079
    Sample:
    Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 ug
    Lane 2: Cerebrum (Rat) Tissue Lysate at 40 ug
    Lane 3: Skin (Rat) Tissue Lysate at 40 ug
    Lane 4: Huvec (Human) Cell Lysate at 30 ug
    Lane 5: HepG2 (Human) Cell Lysate at 30 ug
    Lane 6: THP-1 (Human) Cell Lysate at 30 ug
    Primary: Anti-FENS1 (bs-13169R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 46 kD
    Observed band size: 48 kD
    detail
  • IHC-P - FENS1 Polyclonal Antibody AP55079
    Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FENS1) Polyclonal Antibody, Unconjugated (bs-13169R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF, ICC
Primary Accession Q8IWB7
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 46324 Da
Additional Information
Gene ID 57590
Other Names WD repeat and FYVE domain-containing protein 1, FYVE domain-containing protein localized to endosomes 1, FENS-1, Phosphoinositide-binding protein 1, WD40- and FYVE domain-containing protein 1, Zinc finger FYVE domain-containing protein 17, WDFY1
Dilution Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name WDFY1
Function Positively regulates TLR3- and TLR4-mediated signaling pathways by bridging the interaction between TLR3 or TLR4 and TICAM1. Promotes TLR3/4 ligand-induced activation of transcription factors IRF3 and NF-kappa-B, as well as the production of IFN-beta and inflammatory cytokines (PubMed:25736436).
Cellular Location Early endosome
Research Areas
Citations (0)
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$ 350.00
Cat# AP55079
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Availability: 2 weeks
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