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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   FOXRED2 Polyclonal Antibody   

FOXRED2 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - FOXRED2 Polyclonal Antibody AP55091
    Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-FOXRED2 Polyclonal Antibody, Unconjugated(bs-13210R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • IHC-P - FOXRED2 Polyclonal Antibody AP55091
    Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-FOXRED2 Polyclonal Antibody, Unconjugated(bs-13210R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • FC - FOXRED2 Polyclonal Antibody AP55091
    Blank control:Mouse kidney.
    Primary Antibody (green line): Rabbit Anti-FOXRED2 antibody (bs-13210R)
    Dilution: 2 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-AF488
    Dilution: 1 µg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q8IWF2
Reactivity Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 77791 Da
Additional Information
Gene ID 80020
Other Names FAD-dependent oxidoreductase domain-containing protein 2, Endoplasmic reticulum flavoprotein associated with degradation, FOXRED2 (HGNC:26264)
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name FOXRED2 (HGNC:26264)
Function Probable flavoprotein which may function in endoplasmic reticulum associated degradation (ERAD). May bind non-native proteins in the endoplasmic reticulum and target them to the ubiquitination machinery for subsequent degradation.
Cellular Location Endoplasmic reticulum lumen {ECO:0000255|PROSITE- ProRule:PRU10138, ECO:0000269|PubMed:19706418}
Research Areas
Citations (0)
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$ 350.00
Cat# AP55091
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