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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PCDHB10 Polyclonal Antibody   

PCDHB10 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - PCDHB10 Polyclonal Antibody AP55256
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-PCDHB10 Polyclonal Antibody, Unconjugated(bs-13725R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • IHC-P - PCDHB10 Polyclonal Antibody AP55256
    Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-PCDHB10 Polyclonal Antibody, Unconjugated(bs-13725R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • IHC-P - PCDHB10 Polyclonal Antibody AP55256
    Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCDHB10) Polyclonal Antibody, Unconjugated (bs-13725R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • WB - PCDHB10 Polyclonal Antibody AP55256
    Sample:
    A549(Human) Cell Lysate at 30 ug
    Primary: Anti-PCDHB10 (bs-13725R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 84 kD
    Observed band size: 112 kD
    detail
  • WB - PCDHB10 Polyclonal Antibody AP55256
    Sample:
    Hela(Human) Cell Lysate at 30 ug
    Primary: Anti-PCDHB10 (bs-13725R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 84 kD
    Observed band size: 112 kD
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q9UN67
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 87621 Da
Additional Information
Gene ID 56126
Other Names Protocadherin beta-10, PCDH-beta-10, PCDHB10
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name PCDHB10
Function Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas
Citations (0)
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$ 350.00
Cat# AP55256
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