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Centaurin alpha 2 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Centaurin alpha 2 Polyclonal Antibody AP55315
    Sample:
    MCF-7 Cell (Human) Lysate at 30 ug
    Primary: Anti-Centaurin alpha 2 (bs-13841R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 44kD
    Observed band size: 44kD
    detail
  • IHC-P - Centaurin alpha 2 Polyclonal Antibody AP55315
    Paraformaldehyde-fixed, paraffin embedded (Rat adrenal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Centaurin alpha 2) Polyclonal Antibody, Unconjugated (bs-13841R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q9NPF8
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 44349 Da
Additional Information
Gene ID 55803
Other Names Arf-GAP with dual PH domain-containing protein 2, Centaurin-alpha-2, Cnt-a2, ADAP2, CENTA2
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ADAP2
Synonyms CENTA2
Function GTPase-activating protein for the ADP ribosylation factor family (Potential). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Possesses a stoichiometry of two binding sites for InsP4 with identical affinity.
Cellular Location Cytoplasm. Cell membrane. Note=Constitutively associated with the plasma membrane. Excluded from the nucleus
Tissue Location Highly expressed in placenta, spleen, kidney, skeletal muscle and adrenal gland. Weakly expressed in thyroid, liver, heart, lung, small intestine, peripheral blood leukocytes. Not detected in spinal cord, brain, stomach, trachea, colon, lymph node and bone marrow.
Research Areas
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$ 350.00
Cat# AP55315
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