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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CHST7 Polyclonal Antibody   

CHST7 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CHST7 Polyclonal Antibody AP55362
    Sample:
    Kidney (Mouse) Lysate at 40 ug
    Cerebrum (Mouse) Lysate at 40 ug
    Primary: Anti- CHST7 (bs-13968R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 54 kD
    Observed band size: 54 kD
    detail
  • IHC-P - CHST7 Polyclonal Antibody AP55362
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHST7) Polyclonal Antibody, Unconjugated (bs-13968R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • WB - CHST7 Polyclonal Antibody AP55362
    Sample:
    Ovary (Mouse) Lysate at 40 ug
    Primary: Anti- CHST7 (bs-13968R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 54 kD
    Observed band size: 54 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q9NS84
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 54266 Da
Additional Information
Gene ID 56548
Other Names Carbohydrate sulfotransferase 7, 2.8.2.-, 2.8.2.17, Chondroitin 6-sulfotransferase 2, C6ST-2, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5, GST-5, N-acetylglucosamine 6-O-sulfotransferase 4, GlcNAc6ST-4, Gn6st-4, CHST7
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name CHST7
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc.
Cellular Location Golgi apparatus membrane; Single- pass type II membrane protein
Tissue Location Widely expressed. Highly expressed in heart, spleen, liver and ovary. Expressed at lower level in brain, placenta, thyroid, spinal cord and peripheral blood leukocytes. Not expressed in adult skin.
Research Areas
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$ 350.00
Cat# AP55362
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