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CRB1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - CRB1 Polyclonal Antibody AP55395
    Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRB1) Polyclonal Antibody, Unconjugated (bs-14045R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF, ICC
Primary Accession P82279
Host Rabbit
Clonality Polyclonal
Calculated MW 154183 Da
Additional Information
Gene ID 23418
Other Names Protein crumbs homolog 1, CRB1 (HGNC:2343)
Dilution Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name CRB1 (HGNC:2343)
Function Plays a role in photoreceptor morphogenesis in the retina (By similarity). May maintain cell polarization and adhesion (By similarity).
Cellular Location [Isoform 1]: Apical cell membrane {ECO:0000250|UniProtKB:Q8VHS2}; Single-pass type I membrane protein. Secreted. Cell projection, cilium, photoreceptor outer segment {ECO:0000250|UniProtKB:Q8VHS2} Photoreceptor inner segment {ECO:0000250|UniProtKB:Q8VHS2}
Tissue Location Preferential expression in retina, also expressed in brain, testis, fetal brain and fetal eye (PubMed:15914641) Expressed at the outer limiting membrane and apical to adherens junctions in the retina (PubMed:15914641)
Research Areas
Citations (0)
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$ 350.00
Cat# AP55395
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