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DHDH Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DHDH Polyclonal Antibody AP55512
    Protein: liver(mouse) lysate at 30ug;
    Primary: rabbit Anti-DHDH (bs-14302R) at 1:300;
    Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
    Predicted band size: 36 kD
    Observed band size: 36 kD
    detail
  • IHC-P - DHDH Polyclonal Antibody AP55512
    Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DHDH) Polyclonal Antibody, Unconjugated (bs-14302R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession Q9UQ10
Reactivity Rat, Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 36 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human DHDH
Epitope Specificity 281-334/334
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY Belongs to the Gfo/Idh/MocA family.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions This gene encodes an enzyme that belongs to the family of dihydrodiol dehydrogenases, which exist in multiple forms in mammalian tissues and are involved in the metabolism of xenobiotics and sugars. These enzymes catalyze the NADP1-linked oxidation of transdihydrodiols of aromatic hydrocarbons to corresponding catechols. This enzyme is a dimeric dihydrodiol dehydrogenase, and it differs from monomeric dihydrodiol dehydrogenases in its high substrate specificity for trans-dihydrodiols of aromatic hydrocarbons in the oxidative direction. [provided by RefSeq, Jul 2008]
Additional Information
Gene ID 27294
Other Names Trans-1, 2-dihydrobenzene-1, 2-diol dehydrogenase, 1.3.1.20, D-xylose 1-dehydrogenase, D-xylose-NADP dehydrogenase, 1.1.1.179, Dimeric dihydrodiol dehydrogenase, Hum2DD, DHDH, 2DD
Target/Specificity Small intestine.
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name DHDH
Synonyms 2DD
Tissue Location Small intestine..
Research Areas
Citations (0)
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$ 385.00
Cat# AP55512
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