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DRP2 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - DRP2 Polyclonal Antibody AP55571
    Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DRP2) Polyclonal Antibody, Unconjugated (bs-14428R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • WB - DRP2 Polyclonal Antibody AP55571
    Sample:
    Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 ug
    Lane 2: Cerebrum (Rat) Tissue Lysate at 40 ug
    Lane 3: BV-2 (Mouse) Cell Lysate at 30 ug
    Lane 4: Huvec (Human) Cell Lysate at 30 ug
    Lane 5: Molt-4 (Human) Cell Lysate at 30 ug
    Lane 6: SH-SY5Y (Human) Cell Lysate at 30 ug
    Primary: Anti-DRP2 (bs-14428R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 110 kD
    Observed band size: 120 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF, ICC
Primary Accession Q13474
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 107962 Da
Additional Information
Gene ID 1821
Other Names Dystrophin-related protein 2, DRP-2, DRP2
Dilution Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name DRP2
Function Required for normal myelination and for normal organization of the cytoplasm and the formation of Cajal bands in myelinating Schwann cells. Required for normal PRX location at appositions between the abaxonal surface of the myelin sheath and the Schwann cell plasma membrane. Possibly involved in membrane-cytoskeleton interactions of the central nervous system.
Cellular Location Postsynaptic density {ECO:0000250|UniProtKB:Q9EPA0}. Cell projection, dendrite {ECO:0000250|UniProtKB:Q9EPA0}. Perikaryon {ECO:0000250|UniProtKB:Q9EPA0}. Cell membrane {ECO:0000250|UniProtKB:Q05AA6}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q05AA6}. Note=Detected in Schwann cells at periaxonal myelin membranes. {ECO:0000250|UniProtKB:Q05AA6}
Tissue Location Detected in fetal brain.
Research Areas
Citations (0)
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$ 350.00
Cat# AP55571
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