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DUSP18 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - DUSP18 Polyclonal Antibody AP55582
    Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DUSP18) Polyclonal Antibody, Unconjugated (bs-14453R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF, ICC
Primary Accession Q8NEJ0
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 21066 Da
Additional Information
Gene ID 150290
Other Names Dual specificity protein phosphatase 18, 3.1.3.16, 3.1.3.48, Low molecular weight dual specificity phosphatase 20, LMW-DSP20, DUSP18, LMWDSP20
Dilution IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name DUSP18
Synonyms LMWDSP20
Function Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. In vitro, dephosphorylates p-nitrophenyl phosphate (pNPP).
Cellular Location Cytoplasm. Nucleus. Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q8VE01}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8VE01}; Intermembrane side {ECO:0000250|UniProtKB:Q8VE01}. Note=Translocates to cytoplasm in response to apoptotic stimuli such as staurosporine treatment {ECO:0000250|UniProtKB:Q8VE01}
Tissue Location Widely expressed with highest levels in liver, brain, ovary and testis.
Research Areas
Citations (0)
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$ 350.00
Cat# AP55582
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