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DYRK4 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - DYRK4 Polyclonal Antibody AP55591
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DYRK4) Polyclonal Antibody, Unconjugated (bs-14474R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • WB - DYRK4 Polyclonal Antibody AP55591
    Sample:
    U937(Human) Cell Lysate at 30 ug
    Primary: Anti- DYRK4 (bs-14474R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 60 kD
    Observed band size: 60 kD
    detail
  • WB - DYRK4 Polyclonal Antibody AP55591
    Sample:
    U2OS(Human) Cell Lysate at 30 ug
    MCF-7(Human) Cell Lysate at 30 ug
    HL60(Human) Cell Lysate at 30 ug
    Primary: Anti- DYRK4 (bs-14474R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 60 kD
    Observed band size: 60 kD
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession Q9NR20
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 60 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human DYRK4
Epitope Specificity 31-130/520
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Cytoplasm.
SIMILARITY Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. Contains 1 protein kinase domain.
Post-translational modifications Autophosphorylated on tyrosine residues.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions This gene encodes an enzyme that belongs to a conserved family of serine/threonine protein kinases. Members of this dual specificity kinase family are thought to function in the regulation of cell differentiation and proliferation, survival, and in development. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]
Additional Information
Gene ID 8798
Other Names Dual specificity tyrosine-phosphorylation-regulated kinase 4, 2.7.12.1, DYRK4
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name DYRK4
Function Possible non-essential role in spermiogenesis.
Cellular Location [Isoform 1]: Cytoplasm
Research Areas
Citations (0)
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$ 385.00
Cat# AP55591
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Availability: 4 weeks
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