SIA8C Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, E |
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Primary Accession | O43173 |
Other Accession | Q64689, NP_056963.2 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 43970 Da |
Antigen Region | 312-340 aa |
Gene ID | 51046 |
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Other Names | Sia-alpha-2, 3-Gal-beta-1, 4-GlcNAc-R:alpha 2, 8-sialyltransferase, 2499-, Alpha-2, 8-sialyltransferase 8C, Alpha-2, 8-sialyltransferase III, ST8 alpha-N-acetyl-neuraminide alpha-2, 8-sialyltransferase 3, Sialyltransferase 8C, SIAT8-C, Sialyltransferase St8Sia III, ST8SiaIII, ST8SIA3, SIAT8C |
Target/Specificity | This SIA8C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-340 amino acids from the C-terminal region of human SIA8C. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SIA8C Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ST8SIA3 (HGNC:14269) |
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Synonyms | SIAT8C |
Function | Catalyzes the transfer of sialic acid from a CMP-linked sialic acid donor onto a terminal alpha-2,3-, alpha-2,6-, or alpha-2,8- linked sialic acid of an acceptor, such as N-linked oligosaccharides of glycoproteins and glycolipids through alpha-2,8-linkages (PubMed:9826427, PubMed:26192331, PubMed:10766765). Forms oligosialic and polysialic acid on various sialylated N-acetyllactosamine oligosaccharides of glycoproteins, including FETUB N-glycans, a2-HS- glycoprotein (AHSG) and alpha 2,3-sialylated glycosphingolipids, such as alpha 2,3-sialylparagloboside and ganglioside GM3 and to a lesser extent NCAM1 N-glycans (PubMed:9826427, PubMed:10766765). However, it is much more specific to N-linked oligosaccharides of glycoproteins than glycosphingolipids (By similarity). 2,3-sialylparagloboside serves as the best acceptor substrate among the glycolipids (By similarity). alpha-Neu5Ac-(2->8)-alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-6S-D-GlcNAc and monosialyl and disialyl N-acetyllactosamines are the best acceptor substrates among glycoproteins (PubMed:26192331, PubMed:10766765). May plays critical role in the striatum by mediating the formation of disialylated and trisialylated terminal glycotopes on N- and O-glycans of specific striatal proteins, regulating their distribution in lipid rafts, affecting their interaction with other binding partners, and subsequently modulating striatal functions (By similarity). |
Cellular Location | Golgi apparatus membrane; Single-pass type II membrane protein |
Tissue Location | Expressed in fetal and adult brain and fetal liver. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ST8SIA3 belongs to a family of sialyltransferases that form sialyl-alpha-2,8-sialyl-R linkages at the nonreducing termini of glycoconjugates (Lee et al., 1998 [PubMed 9826427]).
References
Fellay, J., et al. PLoS Genet. 5 (12), E1000791 (2009) :
Kim, S.J., et al. Biochem. Biophys. Res. Commun. 344(4):1057-1064(2006)
Angata, K., et al. J. Biol. Chem. 275(24):18594-18601(2000)
Lee, Y.C., et al. Arch. Biochem. Biophys. 360(1):41-46(1998)
Zeng, G., et al. Gene 187(1):131-134(1997)
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