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IRAK1BP1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - IRAK1BP1 Polyclonal Antibody AP56361
    Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-IRAK1BP1 Polyclonal Antibody, Unconjugated(bs-16694R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • WB - IRAK1BP1 Polyclonal Antibody AP56361
    Protein: MCF-7(human) lyates at 40ug;
    Primary: Rabbit Anti-IRAK1BP1 (bs-16694R) at 1:300;
    Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
    Predicted band size:29 kD Observed band size:33 kD
    detail
  • WB - IRAK1BP1 Polyclonal Antibody AP56361
    Sample:
    Brain (Mouse) Lysate at 40 ug
    Primary: Anti-IRAK1BP1 (bs-16694R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 29 kD
    Observed band size: 29 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q5VVH5
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 29106 Da
Additional Information
Gene ID 134728
Other Names Interleukin-1 receptor-associated kinase 1-binding protein 1, IRAK1-binding protein 1, IRAK1BP1
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name IRAK1BP1
Function Component of the IRAK1-dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. Acts by enhancing RELA transcriptional activity (By similarity).
Cellular Location Cytoplasm. Nucleus.
Research Areas
Citations (0)
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$ 350.00
Cat# AP56361
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