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TRMT1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - TRMT1 Polyclonal Antibody AP56575
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRMT1) Polyclonal Antibody, Unconjugated (bs-17142R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession Q9NXH9
Reactivity Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 72 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human TRMT1
Epitope Specificity 501-600/659
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY Contains 1 C3H1-type zinc finger.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames. N(2),N(2)-dimethylguanosine tRNA methyltransferase, also known as TRMT1 or tRNA(guanine-26,N(2)-N(2)) methyltransferase, is a 659 amino acid enzyme that is responsible for tRNA modifications in eukaryotes. Using S-adenosyl-L-methionine as a methyl donor, TRMT1 dimethylates a single guanine residue at position 26 of tRNA. TRMT1, which was initially identified in yeast and C. elegans, has a 26% and 31% sequence identity to its yeast and C. elegans homologs, respectively. There are two isoforms of TRMT1 produced by alternative splicing events. The TRMT1 gene maps to chromosome 19p13.13 and mutations in this gene lead to abrogated enzyme activity and a decrease in protein levels.
Additional Information
Gene ID 55621
Other Names tRNA (guanine(26)-N(2))-dimethyltransferase, 2.1.1.216, tRNA 2, 2-dimethylguanosine-26 methyltransferase, tRNA(guanine-26, N(2)-N(2)) methyltransferase, tRNA(m(2, 2)G26)dimethyltransferase, TRMT1
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name TRMT1 {ECO:0000303|PubMed:26308914, ECO:0000312|HGNC:HGNC:25980}
Function Dimethylates a single guanine residue at position 26 of most nuclear- and mitochondrial-encoded tRNAs using S-adenosyl-L-methionine as donor of the methyl groups (PubMed:10982862, PubMed:28784718, PubMed:37204604, PubMed:39786990). tRNA guanine(26)-dimethylation is required for redox homeostasis and ensure proper cellular proliferation and oxidative stress survival (PubMed:28784718).
Cellular Location [Isoform 1]: Mitochondrion
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$ 385.00
Cat# AP56575
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