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OTUD1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - OTUD1 Polyclonal Antibody AP56744
    Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (bs-17563R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
  • WB - OTUD1 Polyclonal Antibody AP56744
    Sample: 293T (Human)Cell Lysate at 40 ug
    Primary: Anti-OTUD1(bs-17563R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
    Predicted band size: 51kD
    Observed band size: 60kD
    detail
  • WB - OTUD1 Polyclonal Antibody AP56744
    Sample: HepG2 (Human) Cell Lysate at 40 ug
    Primary: Anti-OTUD1(bs-17563R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
    Predicted band size: 51kD
    Observed band size: 60kD
    detail
  • WB - OTUD1 Polyclonal Antibody AP56744
    Sample: Testis (Mouse)Lysate at 40 ug
    Primary: Anti-OTUD1(bs-17563R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
    Predicted band size: 51kD
    Observed band size: 60kD
    detail
  • IHC-P - OTUD1 Polyclonal Antibody AP56744
    Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (bs-17563R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - OTUD1 Polyclonal Antibody AP56744
    Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (bs-17563R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q5VV17
Reactivity Rat, Cow
Host Rabbit
Clonality Polyclonal
Calculated MW 51063 Da
Additional Information
Gene ID 220213
Other Names OTU domain-containing protein 1, 3.4.19.12, DUBA-7, OTUD1, DUBA7, OTDC1
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name OTUD1 {ECO:0000303|PubMed:23827681, ECO:0000312|HGNC:HGNC:27346}
Function Deubiquitinating enzyme that specifically hydrolyzes 'Lys- 63'-linked polyubiquitin to monoubiquitin (PubMed:23827681). Required for the stability and translation of a subset mRNAs with a high abundance of rare codons by mediating deubiquitination of 40S ribosomal protein RPS10/eS10, thereby antagonizing ZNF598-mediated 40S ubiquitination (PubMed:36445135). The abundance of rare codons in mRNAs can limit the translation rate and can lead to ribosome collisions that trigger activation of ribosome quality control (RQC) pathway by ZNF598 (PubMed:36445135). OTUD1-mediated deubiquitination prevents activation of the RQC and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PubMed:36445135).
Research Areas
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$ 350.00
Cat# AP56744
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