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> home > Products > Primary Antibodies > Antibody Collections > Catalog Updated > MARCH5 Antibody (N-term)
MARCH5 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P-Leica, E |
|---|---|
| Primary Accession | Q9NX47 |
| Other Accession | Q6GM44, Q3KNM2, Q5ZJ41, Q3ZC24, NP_060294 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Chicken, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 31232 Da |
| Antigen Region | 4-32 aa |
| Gene ID | 54708 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase MARCH5, 632-, Membrane-associated RING finger protein 5, Membrane-associated RING-CH protein V, MARCH-V, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, MARCH5, RNF153 |
| Target/Specificity | This MARCH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human MARCH5. |
| Dilution | WB~~1:1000 IHC-P-Leica~~1:500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MARCH5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MARCHF5 (HGNC:26025) |
|---|---|
| Synonyms | MARCH5, RNF153 |
| Function | Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission and as an important regulator of immune response (PubMed:16874301, PubMed:17606867, PubMed:26246171, PubMed:31881323). Plays a crucial role in maintaining mitochondrial homeostasis by regulating the dynamics of mitochondria through the ubiquitination of key proteins involved in fission and fusion such as FIS1, DNM1L and MFN1 (PubMed:16874301, PubMed:17606867). Acts as a critical determinant of mitotic apoptosis through both MCL1- dependent and -independent pathways (By similarity). Turns off persistent immune signaling by degrading oligomeric complexes of retinoic acid-inducible gene I/DDX58 and mitochondrial antiviral- signaling protein/MAVS formed upon RNA virus infection (PubMed:26246171, PubMed:31881323). Promotes STING-mediated type-I interferon production via 'Lys-63'-linked ubiquitination of STING1 thereby preserving its activity and preventing the formation of inactive STING1 polymers (PubMed:37916870). Plays also an essential role in the formation of PEX3-containing vesicles in the de novo biogenesis of peroxisomes from mitochondria (PubMed:39423820, PubMed:39423819). Acts as a regulator of NLRP3 inflammasome activation on the mitochondria by mediating the 'Lys-27'-linked polyubiquitination of NLRP3, positively regulating the NLRP3-NEK7 complex formation and NLRP3 oligomerization (PubMed:37575012). |
| Cellular Location | Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Peroxisome membrane. Note=Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301). |
| Tissue Location | Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle. |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).
References
Yonashiro, R., et al. Mol. Biol. Cell 20(21):4524-4530(2009) Karbowski, M., et al. J. Cell Biol. 178(1):71-84(2007) Nakamura, N., et al. EMBO Rep. 7(10):1019-1022(2006)
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$ 385.00
$ 150.00
Cat# AP5677a
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