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MARCH5 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P-Leica, E |
|---|---|
| Primary Accession | Q9NX47 |
| Other Accession | Q6GM44, Q3KNM2, Q5ZJ41, Q3ZC24, NP_060294 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Chicken, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 31232 Da |
| Antigen Region | 4-32 aa |
| Gene ID | 54708 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase MARCH5, 632-, Membrane-associated RING finger protein 5, Membrane-associated RING-CH protein V, MARCH-V, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, MARCH5, RNF153 |
| Target/Specificity | This MARCH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human MARCH5. |
| Dilution | WB~~1:1000 IHC-P-Leica~~1:500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MARCH5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MARCHF5 (HGNC:26025) |
|---|---|
| Synonyms | MARCH5, RNF153 |
| Function | Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1. |
| Cellular Location | Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301) |
| Tissue Location | Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).
References
Yonashiro, R., et al. Mol. Biol. Cell 20(21):4524-4530(2009) Karbowski, M., et al. J. Cell Biol. 178(1):71-84(2007) Nakamura, N., et al. EMBO Rep. 7(10):1019-1022(2006)
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