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WNT5A Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P-Leica, FC, E |
|---|---|
| Primary Accession | P41221 |
| Other Accession | Q9QXQ7, Q27Q52, P22725, NP_003383.2 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Rabbit |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 42339 Da |
| Antigen Region | 185-213 aa |
| Gene ID | 7474 |
|---|---|
| Other Names | Protein Wnt-5a, WNT5A |
| Target/Specificity | This WNT5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-213 amino acids from the Central region of human WNT5A. |
| Dilution | WB~~1:500 IHC-P~~1:50~100 IHC-P-Leica~~1:100 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | WNT5A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | WNT5A |
|---|---|
| Function | Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta- catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity). |
| Cellular Location | Secreted, extracellular space, extracellular matrix. Secreted |
| Tissue Location | Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level) (PubMed:15735754). Detected in neonate heart and lung (PubMed:8288227) |
Provided below are standard protocols that you may find useful for product applications.
Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq].
References
He, X., et al. Science 275(5306):1652-1654(1997)
Danielson, K.G., et al. J. Biol. Chem. 270(52):31225-31234(1995)
Smolich, B.D., et al. Mol. Biol. Cell 4(12):1267-1275(1993)
Clark, C.C., et al. Genomics 18(2):249-260(1993)
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