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PDLIM3 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF, E
Primary Accession Q53GG5
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 39 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human PDLIM3
Epitope Specificity 301-364/364
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Cytoplasm, myofibril, sarcomere, Z line. Note=Localizes to myofiber Z-lines.
SIMILARITY Contains 1 LIM zinc-binding domain.Contains 1 PDZ (DHR) domain.
SUBUNIT Interacts with ACTN2.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Aberrant alternative splicing of this gene may play a role in myotonic dystrophy.
Additional Information
Gene ID 27295
Other Names PDZ and LIM domain protein 3, Actinin-associated LIM protein, Alpha-actinin-2-associated LIM protein, PDLIM3, ALP
Target/Specificity Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.
Dilution IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name PDLIM3
Synonyms ALP
Function May play a role in the organization of actin filament arrays within muscle cells.
Cellular Location Cytoplasm, myofibril, sarcomere, Z line. Note=Localizes to myofiber Z-lines
Tissue Location Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific
Research Areas
Citations (0)
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$ 385.00
Cat# AP58062
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