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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Phospholipase C beta 2 Polyclonal Antibody   

Phospholipase C beta 2 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - Phospholipase C beta 2 Polyclonal Antibody AP58450
    Blank control: Hela Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
    Primary Antibody: Rabbit Anti-Phospholipase C beta 2/AF647 Conjugated antibody (bs-6472R-AF647), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
    detail
  • WB - Phospholipase C beta 2 Polyclonal Antibody AP58450
    Sample:
    HL-60(Human) Cell Lysate at 30 ug
    Primary: Anti- Phospholipase C beta 2 (bs-6472R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 130 kD
    Observed band size: 130 kD
    detail
  • IHC-P - Phospholipase C beta 2 Polyclonal Antibody AP58450
    Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (bs-6472R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - Phospholipase C beta 2 Polyclonal Antibody AP58450
    Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (bs-6472R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - Phospholipase C beta 2 Polyclonal Antibody AP58450
    Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (bs-6472R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • WB - Phospholipase C beta 2 Polyclonal Antibody AP58450
    Sample:
    K562(Human) Cell Lysate at 30 ug
    Primary: Anti-Phospholipase C beta 2 (bs-6472R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 130 kD
    Observed band size: 130 kD
    detail
  • IF - Phospholipase C beta 2 Polyclonal Antibody AP58450
    Paraformaldehyde-fixed, paraffin embedded (Mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospholipase C beta 2) Polyclonal Antibody, Unconjugated (bs-6472R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q00722
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 134024 Da
Additional Information
Gene ID 5330
Other Names 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase beta-2, 3.1.4.11, Phosphoinositide phospholipase C-beta-2, Phospholipase C-beta-2, PLC-beta-2, PLCB2 (HGNC:9055)
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1 µg/Test,IF=1:50-200,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name PLCB2 (HGNC:9055)
Function The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Research Areas
Citations (0)
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$ 350.00
Cat# AP58450
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