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ICAM5 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ICAM5 Polyclonal Antibody AP58488
    Sample:
    Hippocampus (Mouse) Lysate at 40 ug
    Primary: Anti- ICAM5 (bs-6686R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 97 kD
    Observed band size: 167 kD
    detail
  • FC - ICAM5 Polyclonal Antibody AP58488
    Blank control: Hela.
    Primary Antibody (green line): Rabbit Anti-ICAM5 antibody (bs-6686R)
    Dilution: 1 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 0.2 µg /test.
    Protocol
    The cells were incubated in 5 %BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q9UMF0
Reactivity Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 97116 Da
Additional Information
Gene ID 7087
Other Names Intercellular adhesion molecule 5, ICAM-5, Telencephalin, ICAM5, TLCN, TLN
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=0.2ug/test,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ICAM5
Synonyms TLCN, TLN
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2).
Cellular Location Membrane; Single-pass type I membrane protein.
Tissue Location Expressed on neurons in the most rostral segment of the mammalian brain, the telencephalon
Research Areas
Citations (0)
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$ 350.00
Cat# AP58488
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