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Transglutaminase-1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - Transglutaminase-1 Polyclonal Antibody AP58491
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Transglutaminase Polyclonal Antibody, Unconjugated(bs-6690R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • FC - Transglutaminase-1 Polyclonal Antibody AP58491
    Blank control: Hela.
    Primary Antibody (green line): Rabbit Anti-Transglutaminase antibody (bs-6690R)
    Dilution: 1 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 0.2 µg /test.
    Protocol
    The cells were incubated in 5 %BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF
Primary Accession P22735
Reactivity Rat, Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 89787 Da
Additional Information
Gene ID 7051
Other Names Protein-glutamine gamma-glutamyltransferase K, 2.3.2.13, Epidermal TGase, Transglutaminase K, TG(K), TGK, TGase K, Transglutaminase-1, TGase-1, TGM1, KTG
Dilution Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=0.2ug/test,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name TGM1
Synonyms KTG
Function Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Involved in cell proliferation (PubMed:26220141).
Cellular Location Membrane; Lipid- anchor
Research Areas
Citations (0)
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$ 350.00
Cat# AP58491
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