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Netrin G1 ligand Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - Netrin G1 ligand Polyclonal Antibody AP58495
    Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-NGL1 Polyclonal Antibody, Unconjugated(bs-6710R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
    detail
  • IF - Netrin G1 ligand Polyclonal Antibody AP58495
    Tissue/cell: mouse brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-NGL1 Polyclonal Antibody, Unconjugated(bs-6710R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
    detail
  • WB - Netrin G1 ligand Polyclonal Antibody AP58495
    Sample: Hela Cell (Human) Lysate at 40 ug
    Primary: Anti- Netrin G1 ligand (bs-6710R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 70 kD
    Observed band size: 76 kD
    detail
  • WB - Netrin G1 ligand Polyclonal Antibody AP58495
    Sample: BV2 Cell (Mouse) Lysate at 40 ug
    Primary: Anti- Netrin G1 ligand (bs-6710R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 70 kD
    Observed band size: 76 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q9HCJ2
Reactivity Rat, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 71950 Da
Additional Information
Gene ID 57689
Other Names Leucine-rich repeat-containing protein 4C, Netrin-G1 ligand, NGL-1, LRRC4C, KIAA1580, NGL1
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name LRRC4C
Synonyms KIAA1580, NGL1
Function May promote neurite outgrowth of developing thalamic neurons.
Cellular Location Postsynaptic cell membrane; Single-pass type I membrane protein
Tissue Location Highly expressed in the cerebral cortex, including frontal, parietal and occipital lobes. Putamen, amygdala, hippocampus and medulla oblongata show moderate expression. Caudate nucleus and thalamus express small amounts, whereas other brain regions show very weak or no expression.
Research Areas
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$ 350.00
Cat# AP58495
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