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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Porimin Polyclonal Antibody   

Porimin Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Porimin Polyclonal Antibody AP58709
    Sample:
    Skin (Mouse) Lysate at 40 ug
    Skin (Rat) Lysate at 40 ug
    Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 19 kD
    Observed band size: 64 kD
    detail
  • FC - Porimin Polyclonal Antibody AP58709
    Blank control:Molt4.
    Primary Antibody (green line): Rabbit Anti-Porimin antibody (bs-7674R)
    Dilution: 2 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 1 µg /test.
    Protocol
    The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
  • WB - Porimin Polyclonal Antibody AP58709
    Sample:
    Embryo (Mouse) Lysate at 40 ug
    Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 19 kD
    Observed band size: 74 kD
    detail
  • WB - Porimin Polyclonal Antibody AP58709
    Sample:
    Lymph node (Mouse) Lysate at 40 ug
    Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 19 kD
    Observed band size: 74 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q8N131
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 21531 Da
Additional Information
Gene ID 114908
Other Names Porimin, Keratinocytes-associated transmembrane protein 3, KCT-3, Pro-oncosis receptor inducing membrane injury, Transmembrane protein 123, TMEM123, KCT3
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name TMEM123
Synonyms KCT3
Function Implicated in oncotic cell death, characterized by cell swelling, organelle swelling, vacuolization and increased membrane permeability.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Ubiquitous. Not expressed in ovary. Expressed in keratinocytes.
Research Areas
Citations (0)
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$ 350.00
Cat# AP58709
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