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CCDC94 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CCDC94 Polyclonal Antibody AP58861
    Sample:
    Testis (Mouse) Lysate at 40 ug
    Primary: Anti- CCDC94 (bs-8081R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 37 kD
    Observed band size: 35 kD
    detail
  • IHC-P - CCDC94 Polyclonal Antibody AP58861
    Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-CCDC94 Polyclonal Antibody, Unconjugated(bs-8081R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q9BW85
Reactivity Rat, Pig, Dog, Cow
Host Rabbit
Clonality Polyclonal
Calculated MW 37086 Da
Additional Information
Gene ID 55702
Other Names Splicing factor YJU2 {ECO:0000255|HAMAP-Rule:MF_03226}, Coiled-coil domain-containing protein 94, YJU2 {ECO:0000255|HAMAP-Rule:MF_03226, ECO:0000312|HGNC:HGNC:25518}
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name YJU2 {ECO:0000255|HAMAP-Rule:MF_03226, ECO:0000312|HGNC:HGNC:25518}
Function Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA (PubMed:29301961). Plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'- intermediates (By similarity). May protect cells from TP53-dependent apoptosis upon dsDNA break damage through association with PRP19-CD5L complex (PubMed:22952453).
Cellular Location Nucleus {ECO:0000255|HAMAP-Rule:MF_03226, ECO:0000269|PubMed:29301961}
Research Areas
Citations (0)
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$ 350.00
Cat# AP58861
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