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CCDC8 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CCDC8 Polyclonal Antibody AP58890
    Sample:
    Muscle (Mouse) Lysate at 40 ug
    Primary: Anti- CCDC8 (bs-8138R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 59 kD
    Observed band size: 59 kD
    detail
  • IHC-P - CCDC8 Polyclonal Antibody AP58890
    Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC8) Polyclonal Antibody, Unconjugated (bs-8138R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q9H0W5
Reactivity Rat, Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 59374 Da
Additional Information
Gene ID 83987
Other Names Coiled-coil domain-containing protein 8, CCDC8
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name CCDC8
Function Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed:24793695, PubMed:24793696). Required for localization of CUL7 to the centrosome (PubMed:24793695).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Tissue Location Widely expressed with low levels in spleen, skeletal muscle, small intestine, kidney and liver
Research Areas
Citations (0)
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$ 350.00
Cat# AP58890
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