PENK Antibody (Center)

PENK Antibody (Center) (Cat. #AP5889c) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PENK antibody detected the PENK protein (arrow).
PENK antibody (Center) (Cat. #AP5889c) immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PENK antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
PENK Antibody (Center) (Cat. #AP5889c) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SPECIFICATION
CITATIONS
PROTOCOLS
BACKGROUND

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, IHC-P, FC, E
Primary Accession	P01210
Other Accession	NP_001129162.1, NP_006202.1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	30787 Da
Antigen Region	145-174 aa

Additional Information
Gene ID	5179
Other Names	Proenkephalin-A, Synenkephalin, Met-enkephalin, Opioid growth factor, OGF, PENK(114-133), PENK(143-183), Met-enkephalin-Arg-Gly-Leu, Leu-enkephalin, PENK(237-258), Met-enkephalin-Arg-Phe, PENK
Target/Specificity	This PENK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human PENK.
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PENK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	PENK (HGNC:8831)
Function	[Met-enkephalin]: Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
Cellular Location	Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen {ECO:0000250\|UniProtKB:P01211}. Secreted {ECO:0000250\|UniProtKB:P01211}

Research Areas

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Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

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