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UBE2E2 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - UBE2E2 Polyclonal Antibody AP58973
    Sample: A549 Cell (Human) Lysate at 40 ug
    Primary: Anti-UBE2E2 (bs-8353R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 22 kD
    Observed band size: 20 kD
    detail
  • IHC-P - UBE2E2 Polyclonal Antibody AP58973
    Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBE2E2) Polyclonal Antibody, Unconjugated (bs- 8353R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
  • IHC-P - UBE2E2 Polyclonal Antibody AP58973
    Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBE2E2) Polyclonal Antibody, Unconjugated (bs- 8353R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q96LR5
Reactivity Rat, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 22255 Da
Additional Information
Gene ID 7325
Other Names Ubiquitin-conjugating enzyme E2 E2, 2.3.2.23, E2 ubiquitin-conjugating enzyme E2, UbcH8, Ubiquitin carrier protein E2, Ubiquitin-protein ligase E2, UBE2E2, UBCH8
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name UBE2E2
Synonyms UBCH8
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.
Research Areas
Citations (0)
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$ 350.00
Cat# AP58973
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