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NEU1/Neuraminidase Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NEU1/Neuraminidase Polyclonal Antibody AP59051
    Sample:U251 (human)cell Lysate at 40 ug
    Primary: Anti-NEU1/Neuraminidase(bs-8624R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
    Predicted band size: 45kD
    Observed band size: 45kD
    detail
  • WB - NEU1/Neuraminidase Polyclonal Antibody AP59051
    Sample: A431 Cell (Human) Lysate at 40 ug
    Primary: Anti-NEU1/Neuraminidase (bs-8624R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 45kD
    Observed band size: 45kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q99519
Reactivity Rat, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 45467 Da
Additional Information
Gene ID 4758
Other Names Sialidase-1, 3.2.1.18, Acetylneuraminyl hydrolase, G9 sialidase, Lysosomal sialidase, N-acetyl-alpha-neuraminidase 1, NEU1, NANH
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name NEU1
Synonyms NANH
Function Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.
Cellular Location Lysosome membrane; Peripheral membrane protein; Lumenal side. Lysosome lumen. Cell membrane. Cytoplasmic vesicle Lysosome. Note=Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles
Tissue Location Highly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain.
Research Areas
Citations (0)
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$ 350.00
Cat# AP59051
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