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HEY2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HEY2 Antibody (N-term) AP5914a
    All lanes : Anti-HEY2 Antibody (N-term) at 1:1000 dilution Lane 1: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 41kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - HEY2 Antibody (N-term) AP5914a
    HEY2 Antibody (N-term) (Cat. #AP5914a) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the HEY2 antibody detected HEY2 protein (arrow).
    detail
  • IHC-P - HEY2 Antibody (N-term) AP5914a
    HEY2 antibody (N-term) (Cat. #AP5914a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HEY2 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9UBP5
Other Accession Q7KM13, Q9QUS4, Q9I9L0, Q9I8A3, Q9WV93, Q9Y5J3, Q8AXV6, Q2KIN4, NP_036391.1
Reactivity Human, Mouse
Predicted Bovine, Zebrafish, Xenopus, Drosophila
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35808 Da
Antigen Region 65-91 aa
Additional Information
Gene ID 23493
Other Names Hairy/enhancer-of-split related with YRPW motif protein 2, Cardiovascular helix-loop-helix factor 1, hCHF1, Class B basic helix-loop-helix protein 32, bHLHb32, HES-related repressor protein 2, Hairy and enhancer of split-related protein 2, HESR-2, Hairy-related transcription factor 2, HRT-2, hHRT2, Protein gridlock homolog, HEY2, BHLHB32, CHF1, GRL, HERP, HERP1, HRT2
Target/Specificity This HEY2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-91 amino acids from the N-terminal region of human HEY2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHEY2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HEY2
Synonyms BHLHB32, CHF1, GRL, HERP, HERP1, HRT2
Function Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00380, ECO:0000255|PROSITE-ProRule:PRU00981}
Research Areas
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$ 365.00
$ 140.00
Cat# AP5914a
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