RNF3 Polyclonal Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IHC-F, IF |
---|---|
Primary Accession | Q3KNV8 |
Reactivity | Rat, Pig, Dog, Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 28115 Da |
Gene ID | 10336 |
---|---|
Other Names | Polycomb group RING finger protein 3, RING finger protein 3A, PCGF3, RNF3, RNF3A |
Dilution | WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200, |
Format | 0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce |
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃. |
Name | PCGF3 |
---|---|
Synonyms | RNF3, RNF3A |
Function | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332). Plays a redundant role with PCGF5 as part of a PRC1- like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (By similarity). |
Cellular Location | Nucleus. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q8BTQ0}. Note=Recruited by the non- coding RNA Xist to specific nuclear foci that probably correspond to the inactivated X chromosome. {ECO:0000250|UniProtKB:Q8BTQ0} |
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