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RNF3 Polyclonal Antibody
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | Q3KNV8 |
| Reactivity | Rat, Pig, Dog, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 28 KDa |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human PCGF3/RNF3 |
| Epitope Specificity | 141-242/242 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Nucleus. |
| SIMILARITY | Contains 1 RING-type zinc finger. |
| SUBUNIT | Component of a PRC1-like complex. Interacts with CBX6, CBX7 and CBX8. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Polycomb group (PcG) proteins form multiprotein complexes that regulate expression patterns of developmental and cell proliferation genes. Several members of the PcG contain ring finger domains and are identified as a subclass of RING finger proteins. The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain the RING-type zinc finger conserved domain are generally involved in the ubiquitination pathway of protein degradation. PCGF3 (polycomb group ring finger 3), also known as RNF3, DONG1 or RNF3A, is a 242 amino acid transcriptional regulator that is encoded by a gene located on human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. PCGF3 exists as two isoforms produced by alternative splicing events. |
| Gene ID | 10336 |
|---|---|
| Other Names | Polycomb group RING finger protein 3, RING finger protein 3A, PCGF3, RNF3, RNF3A |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000 |
| Format | 0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce |
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃. |
| Name | PCGF3 |
|---|---|
| Synonyms | RNF3, RNF3A |
| Function | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332). Plays a redundant role with PCGF5 as part of a PRC1- like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (By similarity). |
| Cellular Location | Nucleus. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q8BTQ0}. Note=Recruited by the non- coding RNA Xist to specific nuclear foci that probably correspond to the inactivated X chromosome. {ECO:0000250|UniProtKB:Q8BTQ0} |
Research Areas
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Application Protocols
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$ 385.00
Cat# AP59167
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