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MARCH8 Polyclonal Antibody
Purified Rabbit Polyclonal Antibody (Pab)
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- BACKGROUND
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q5T0T0 |
| Reactivity | Rat, Pig, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 32965 Da |
| Gene ID | 220972 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase MARCHF8, 2.3.2.27, Cellular modulator of immune recognition, c-MIR, Membrane-associated RING finger protein 8, Membrane-associated RING-CH protein VIII, MARCH-VIII, RING finger protein 178, RING-type E3 ubiquitin transferase MARCHF8, MARCHF8 (HGNC:23356), MARCH8, MIR, RNF178 |
| Dilution | WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200, |
| Format | 0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce |
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃. |
| Name | MARCHF8 (HGNC:23356) |
|---|---|
| Synonyms | MARCH8, MIR, RNF178 |
| Function | E3 ubiquitin-protein ligase that plays several important roles in innate immunity and adaptive immunity (PubMed:34285233, PubMed:35019698, PubMed:35503863). Mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies (PubMed:19117940, PubMed:19566897). Possesses a very broad antiviral activity by specifically inactivating different viral fusion proteins (PubMed:32934085). Targets and ubiquitinates cytoplasmic lysine residues of viral envelope glycoproteins with single transmembrane domains leading to their lysosomal degradation (PubMed:35019698). Therefore, shows broad-spectrum inhibition against many viruses including retroviruses, rhabdoviruses, arenaviruses, sarbecoviruses or influenzaviruses (PubMed:35019698, PubMed:34285233). Strongly blocks human immunodeficiency virus type 1 envelope glycoprotein incorporation into virions by down-regulating its cell surface expression. Blocks also ebola virus glycoprotein/GP incorporation via surface down-regulation (PubMed:32934085). Mediates 'Lys-63'-linked polyubiquitination of influenza M2 to target it to lysosome for degradation (PubMed:34285233). Mediates the regulation of constitutive ubiquitination and trafficking of the viral restriction factor BST2 within the endocytic pathway (PubMed:28320822). Plays a role in maintenance of immune tolerance to self by promoting the turnover and proteasomal degradation of PD-L1/CD274 via ubiquitination (PubMed:34183449). Catalyzes the 'Lys-63'-linked polyubiquitylation of cGAS thereby inhibiting its DNA binding ability and impairing its antiviral innate immunity (PubMed:35503863). |
| Cellular Location | Golgi apparatus membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein |
| Tissue Location | Broadly expressed. Present in immature dendritic cells (at protein level). |
Research Areas
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Application Protocols
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$ 350.00
Cat# AP59231
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