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ZNF828 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - ZNF828 Polyclonal Antibody AP59303
    Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF828) Polyclonal Antibody, Unconjugated (bs-9607R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
  • IHC-P - ZNF828 Polyclonal Antibody AP59303
    Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF828) Polyclonal Antibody, Unconjugated (bs-9607R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF, ICC, E
Primary Accession Q96JM3
Reactivity Rat, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 89 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human ZNF828/C13orf8
Epitope Specificity 621-720/812
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Nucleus. Chromosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle.
SIMILARITY Contains 1 C2H2-type zinc finger.
SUBUNIT Interacts with MAD2L2. Interacts with POGZ, CBX1, CBX3 and CBX5.
Post-translational modifications Phosphorylated by CDK1. Mitotic phosphorylation is required for the attachment of spindle microtubules to the kinetochore.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF828, is a 812 amino acid protein that contains one C2H2-type zinc finger and is localized to the cytoplasm and the nucleus. The gene encoding ZNF828 maps to chromosome 13. Comprising nearly 4% of the human genome, chromosome 13 contains around 114 million base pairs and encodes over 400 genes. Chromosome 13 houses key tumor suppressor genes, including BRCA2 and RB1, which are associated with breast cancer susceptibility and retinoblastoma, respectively. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections.
Additional Information
Gene ID 283489
Other Names Chromosome alignment-maintaining phosphoprotein 1, Zinc finger protein 828, CHAMP1, C13orf8, CAMP, CHAMP, KIAA1802, ZNF828
Dilution IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name CHAMP1
Synonyms C13orf8, CAMP, CHAMP, KIAA1802, ZNF828
Function Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
Cellular Location Nucleus. Chromosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle
Research Areas
Citations (0)
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$ 385.00
Cat# AP59303
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