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KCNE1L Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - KCNE1L Polyclonal Antibody AP59426
    Blank control: Hela.
    Primary Antibody (green line): Rabbit Anti-KCNE1L antibody (bs-9929R)
    Dilution: 1 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 1 µg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q9UJ90
Reactivity Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 14993 Da
Additional Information
Gene ID 23630
Other Names Potassium voltage-gated channel subfamily E regulatory beta subunit 5, AMME syndrome candidate gene 2 protein, Potassium channel subunit beta MiRP4, Potassium voltage-gated channel subfamily E member 1-like protein, KCNE5, AMMECR2, KCNE1L {ECO:0000303|PubMed:10493825}
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/test,IF=1:50-200,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name KCNE5
Synonyms AMMECR2, KCNE1L {ECO:0000303|PubMed:1049
Function Potassium channel ancillary subunit that is essential for generation of some native K(+) currents by virtue of formation of heteromeric ion channel complex with voltage-gated potassium (Kv) channel pore-forming alpha subunits. Functions as an inhibitory beta- subunit of the repolarizing cardiac potassium ion channel KCNQ1.
Cellular Location Membrane; Single- pass type I membrane protein
Tissue Location Highly expressed in heart, skeletal muscle, brain, spinal cord and placenta.
Research Areas
Citations (0)
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$ 350.00
Cat# AP59426
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