Anti-GAPDH Antibody
Rabbit polyclonal antibody to GAPDH
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q5XJ10 |
Reactivity | Zebrafish |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 35784 Da |
Gene ID | 317743 |
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Other Names | GAPD; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH |
Target/Specificity | Recognizes endogenous levels of GAPDH protein. |
Dilution | WB~~1/2000 - 1/5000 |
Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
Storage | Store at -20 °C.Stable for 12 months from date of receipt |
Name | gapdh {ECO:0000312|ZFIN:ZDB-GENE-030115-1} |
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Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). |
Cellular Location | Cytoplasm, cytosol {ECO:0000250|UniProtKB:P04797}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P04797}. Nucleus {ECO:0000250|UniProtKB:P04797} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Recombinant protein corresponding to zebrafish GAPDH.
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