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Anti-Galactose Kinase Antibody

Rabbit polyclonal antibody to Galactose Kinase

     
  • WB - Anti-Galactose Kinase Antibody AP59563
    Western blot analysis of Galactose Kinase expression in HEK293T (A), Hela (B), mouse lung (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates.
    detail
  • IF - Anti-Galactose Kinase Antibody AP59563
    Immunofluorescent analysis of Galactose Kinase staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession P51570
Other Accession Q9R0N0
Reactivity Human, Mouse, Rat, Drosophila
Host Rabbit
Clonality Polyclonal
Calculated MW 42272 Da
Additional Information
Gene ID 2584
Other Names GALK; Galactokinase; Galactose kinase
Target/Specificity Recognizes endogenous levels of Galactose Kinase protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name GALK1 (HGNC:4118)
Synonyms GALK
Function Catalyzes the transfer of a phosphate from ATP to alpha-D- galactose and participates in the first committed step in the catabolism of galactose.
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Galactose Kinase. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP59563
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