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Anti-NDUFA8 Antibody

Rabbit polyclonal antibody to NDUFA8

     
  • WB - Anti-NDUFA8 Antibody AP59632
    Western blot analysis of NDUFA8 expression in HEK293T (A), Hela (B), A2780 (C), HepG2 (D), mouse muscle (E), mouse kidney (F), rat kidney (G) whole cell lysates.
    detail
  • IH - Anti-NDUFA8 Antibody AP59632
    Immunohistochemical analysis of NDUFA8 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP, IHC
Primary Accession P51970
Other Accession Q9DCJ5
Reactivity Human, Mouse, Rat, Monkey, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 20105 Da
Additional Information
Gene ID 4702
Other Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; Complex I-19kD; CI-19kD; Complex I-PGIV; CI-PGIV; NADH-ubiquinone oxidoreductase 19 kDa subunit
Target/Specificity Recognizes endogenous levels of NDUFA8 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name NDUFA8
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis (PubMed:27626371, PubMed:32385911, PubMed:33153867). Complex I functions in the transfer of electrons from NADH to the respiratory chain (PubMed:27626371). The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed:27626371).
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein. Mitochondrion intermembrane space. Mitochondrion
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NDUFA8. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP59632
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