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Anti-RAD18 Antibody
Rabbit polyclonal antibody to RAD18
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- BACKGROUND
Application 
| WB, IP |
|---|---|
| Primary Accession | Q9NS91 |
| Reactivity | Human, Mouse, Rat, Monkey, Pig |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 56223 Da |
| Gene ID | 56852 |
|---|---|
| Other Names | RNF73; E3 ubiquitin-protein ligase RAD18; Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73 |
| Target/Specificity | Recognizes endogenous levels of RAD18 protein. |
| Dilution | WB~~WB (1/500 - 1/1000), IP (1/10 - 1/100) |
| Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | RAD18 |
|---|---|
| Synonyms | RNF73 |
| Function | E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA- associated PCNA on 'Lys-164'. Has ssDNA binding activity. |
| Cellular Location | Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with chromatin (PubMed:25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607). Accumulates with the SLF1- SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565) |
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Provided below are standard protocols that you may find useful for product applications.
Background
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAD18. The exact sequence is proprietary.
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