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Anti-ATRIP Antibody

Rabbit polyclonal antibody to ATRIP

     
  • WB - Anti-ATRIP Antibody AP60121
    Western blot analysis of ATRIP expression in Jurkat (A) whole cell lysates.
    detail
  • IH - Anti-ATRIP Antibody AP60121
    Immunohistochemical analysis of ATRIP staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-ATRIP Antibody AP60121
    Immunofluorescent analysis of ATRIP staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IF/IC, IHC
Primary Accession Q8WXE1
Other Accession Q8BMG1
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 85838 Da
Additional Information
Gene ID 84126
Other Names AGS1; ATR-interacting protein; ATM and Rad3-related-interacting protein
Target/Specificity Recognizes endogenous levels of ATRIP protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ATRIP
Synonyms AGS1
Function Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
Cellular Location Nucleus. Note=Redistributes to discrete nuclear foci upon DNA damage
Tissue Location Ubiquitous..
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ATRIP. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60121
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