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Anti-GPR116 Antibody

Rabbit polyclonal antibody to GPR116

     
  • WB - Anti-GPR116 Antibody AP60174
    Western blot analysis of GPR116 expression in 3T3L1 (A), PC12 (B), HEK293T (C), A549 (D) whole cell lysates.
    detail
  • IH - Anti-GPR116 Antibody AP60174
    Immunohistochemical analysis of GPR116 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-GPR116 Antibody AP60174
    Immunofluorescent analysis of GPR116 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession Q8IZF2
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 149457 Da
Additional Information
Gene ID 221395
Other Names KIAA0758; Probable G-protein coupled receptor 116
Target/Specificity Recognizes endogenous levels of GPR116 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ADGRF5 (HGNC:19030)
Synonyms GPR116, KIAA0758
Function Receptor that plays a critical role in lung surfactant homeostasis. May play a role in controlling adipocyte function.
Cellular Location Cell membrane {ECO:0000250|UniProtKB:Q9WVT0}; Multi-pass membrane protein
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR116. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60174
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