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>   home   >   Products   >   Primary Antibodies   >   Anti-EEF2 (pT56) Antibody   

Anti-EEF2 (pT56) Antibody

Rabbit polyclonal antibody to EEF2 (pT56)

     
  • WB - Anti-EEF2 (pT56) Antibody AP60272
    Western blot analysis of EEF2 (pT56) expression in DLD (A), U2OS (B) whole cell lysates.
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  • IH - Anti-EEF2 (pT56) Antibody AP60272
    Immunohistochemical analysis of EEF2 (pT56) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-EEF2 (pT56) Antibody AP60272
    Immunofluorescent analysis of EEF2 (pT56) staining in SKOV3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession P13639
Other Accession P58252
Reactivity Human, Mouse, Rat, Zebrafish, Monkey, Chicken, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 95338 Da
Additional Information
Gene ID 1938
Other Names EF2; Elongation factor 2; EF-2
Target/Specificity Recognizes endogenous levels of EEF2 (pT56) protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name EEF2
Synonyms EF2
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed:26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl- tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed:26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed:26593721).
Cellular Location Cytoplasm. Nucleus. Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C- terminal cleavage product.
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EEF2 (pT56). The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60272
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