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Anti-PU.1 Antibody
Rabbit polyclonal antibody to PU.1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF/IC, IHC |
|---|---|
| Primary Accession | P17947 |
| Other Accession | P17433 |
| Reactivity | Human, Mouse, Rat, Zebrafish, Pig, Chicken |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 31083 Da |
| Gene ID | 6688 |
|---|---|
| Other Names | Transcription factor PU.1; 31 kDa-transforming protein |
| Target/Specificity | Recognizes endogenous levels of PU.1 protein. |
| Dilution | WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) |
| Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | SPI1 |
|---|---|
| Function | Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed:33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity). |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00237, ECO:0000269|PubMed:33951726} |
| Tissue Location | In the bone marrow, concentrated in hematopoietic stem cell, lymphoid progenitor, myeloid lineage (granulocyte macrophage progenitors, classical dendritic cells, monocytes) and B-cell clusters Among B-cells, predominantly expressed in pre-B1 cells (PubMed:33951726). Expressed in germinal center B-cells (PubMed:23166356). |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PU.1. The exact sequence is proprietary.
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$ 350.00
$ 175.00
Cat# AP60516
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