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Anti-GPR73a Antibody

Rabbit polyclonal antibody to GPR73a

     
  • WB - Anti-GPR73a Antibody AP60619
    Western blot analysis of GPR73a expression in BV2 (A), H9C2 (B), HCC827 (C), HEK293T (D) whole cell lysates.
    detail
  • IF - Anti-GPR73a Antibody AP60619
    Immunofluorescent analysis of GPR73a staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession Q8TCW9
Other Accession Q9JKL1
Reactivity Human, Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 44770 Da
Additional Information
Gene ID 10887
Other Names GPR73; PKR1; Prokineticin receptor 1; PK-R1; G-protein coupled receptor 73; G-protein coupled receptor ZAQ; GPR73a
Target/Specificity Recognizes endogenous levels of GPR73a protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PROKR1
Synonyms GPR73, PKR1
Function Receptor for prokineticin 1. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. May play a role during early pregnancy.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Localizes to glandular epithelium, stroma and vascular endothelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non- pregnant endometrium. Expressed in the stomach, throughout the small intestine, colon, rectum, thyroid gland, pituitary gland, salivary gland, adrenal gland, testis, ovary, brain, spleen, prostate and pancreas.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR73a. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60619
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