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Anti-Histone H2A.X (pS139) Antibody
Rabbit polyclonal antibody to Histone H2A.X (pS139)
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Application 
| WB, FC, IP, IHC |
|---|---|
| Primary Accession | P16104 |
| Other Accession | P27661 |
| Reactivity | Human, Mouse, Rabbit |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 15145 Da |
| Gene ID | 3014 |
|---|---|
| Other Names | H2AX; Histone H2AX; H2a/x; Histone H2A.X |
| Target/Specificity | Recognizes endogenous levels of Histone H2A.X (pS139) protein. |
| Dilution | WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100), FC (1/100 - 1/200) IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100), FC (1/100 - 1/200) |
| Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | H2AX (HGNC:4739) |
|---|---|
| Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Cellular Location | Nucleus. Chromosome |
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Provided below are standard protocols that you may find useful for product applications.
Background
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Histone H2A.X. The exact sequence is proprietary.
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