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Anti-AAK1 Antibody
Rabbit polyclonal antibody to AAK1
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Application 
| WB, IF/IC, IHC |
|---|---|
| Primary Accession | Q2M2I8 |
| Other Accession | Q3UHJ0 |
| Reactivity | Human, Mouse, Rat, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 103885 Da |
| Gene ID | 22848 |
|---|---|
| Other Names | KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1 |
| Target/Specificity | Recognizes endogenous levels of AAK1 protein. |
| Dilution | WB~~WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100) IH~~WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100) IF~~WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100) |
| Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | AAK1 |
|---|---|
| Synonyms | KIAA1048 |
| Function | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed:17494869, PubMed:11877457, PubMed:11877461, PubMed:12952931, PubMed:14617351, PubMed:25653444). Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1 (PubMed:17494869). Preferentially, may phosphorylate substrates on threonine residues (PubMed:11877457, PubMed:18657069). Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes (PubMed:12952931). Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes (PubMed:18657069). Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (PubMed:21464124). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:F1MH24}; Peripheral membrane protein {ECO:0000250|UniProtKB:F1MH24}. Membrane, clathrin-coated pit. Presynapse {ECO:0000250|UniProtKB:P0C1X8}. Note=Active when found in clathrin- coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells. {ECO:0000250|UniProtKB:P0C1X8} |
| Tissue Location | Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain. |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AAK1. The exact sequence is proprietary.
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$ 350.00
$ 175.00
Cat# AP60850
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