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Anti-GPR66 Antibody

Rabbit polyclonal antibody to GPR66

     
  • WB - Anti-GPR66 Antibody AP60914
    Western blot analysis of GPR66 expression in rat testis (A), mouse testis (B), SGC7901 (C), K562 (D) whole cell lysates.
    detail
  • IF - Anti-GPR66 Antibody AP60914
    Immunofluorescent analysis of GPR66 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession Q9HB89
Other Accession O55040
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 47351 Da
Additional Information
Gene ID 10316
Other Names GPR66; Neuromedin-U receptor 1; NMU-R1; G-protein coupled receptor 66; G-protein coupled receptor FM-3
Target/Specificity Recognizes endogenous levels of GPR66 protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name NMUR1
Synonyms GPR66
Function Receptor for the neuromedin-U and neuromedin-S neuropeptides.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Expressed in greatest abundance in peripheral organs, particularly in elements of the gastrointestinal and urogenital systems with highest levels in testes. In central nervous system structures express levels are much lower than those seen in peripheral organs. Within the CNS, has been detected in highest abundance in the cerebellum, dorsal root ganglia, hippocampus, and spinal cord
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR66. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60914
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