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Anti-Vimentin (pS39) Antibody

Rabbit polyclonal antibody to Vimentin (pS39)

     
  • WB - Anti-Vimentin (pS39) Antibody AP61096
    Western blot analysis of Vimentin (pS39) expression in PMVEC (A), A2780 (B), NIH3T3L1 (C) whole cell lysates.
    detail
  • E - Anti-Vimentin (pS39) Antibody AP61096
    Direct ELISA antibody dose-response curve using Anti-Vimentin (pS39) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P08670
Other Accession P20152
Reactivity Human, Mouse, Rat, Pig, Bovine, SARS
Host Rabbit
Clonality Polyclonal
Calculated MW 53652 Da
Additional Information
Gene ID 7431
Other Names Vimentin
Target/Specificity Recognizes endogenous levels of Vimentin (pS39) protein.
Dilution WB~~WB (1/500 - 1/1000)
E~~WB (1/500 - 1/1000)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Vimentin. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61096
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