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Anti-ERK1/2 (pY204) Antibody

Rabbit polyclonal antibody to ERK1/2 (pY204)

     
  • WB - Anti-ERK1/2 (pY204) Antibody AP61175
    Western blot analysis of ERK1/2 (pY204) expression in HCT116 (A), A549 (B) whole cell lysates.
    detail
  • IH - Anti-ERK1/2 (pY204) Antibody AP61175
    Immunohistochemical analysis of ERK1/2 (pY204) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-ERK1/2 (pY204) Antibody AP61175
    Immunofluorescent analysis of ERK1/2 (pY204) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
  • E - Anti-ERK1/2 (pY204) Antibody AP61175
    Direct ELISA antibody dose-response curve using Anti-ERK1/2 (pY204) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession P27361, P28482
Other Accession Q63844, P63085
Reactivity Human, Mouse, Rat, Zebrafish, Chicken, Bovine
Host Rabbit
Clonality Polyclonal
Additional Information
Other Names MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2
Target/Specificity Recognizes endogenous levels of ERK1/2 (pY204) protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
IH~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
E~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ERK1/2 (pY204). The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61175
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