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Anti-5-HT5A Antibody

Rabbit polyclonal antibody to 5-HT5A

     
  • WB - Anti-5-HT5A Antibody AP61351
    Western blot analysis of 5-HT5A expression in rat kidney (A) whole cell lysates.
    detail
  • IF - Anti-5-HT5A Antibody AP61351
    Immunofluorescent analysis of 5-HT5A staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession P47898
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 40255 Da
Additional Information
Gene ID 3361
Other Names 5-hydroxytryptamine receptor 5A; 5-HT-5; 5-HT-5A; 5-HT5A; Serotonin receptor 5A
Target/Specificity Recognizes endogenous levels of 5-HT5A protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HTR5A
Function This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins.
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human 5-HT5A. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61351
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