Anti-HEXO Antibody
Rabbit polyclonal antibody to HEXO
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q8IV48 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 40064 Da |
Gene ID | 90459 |
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Other Names | 3'EXO; THEX1; 3'-5' exoribonuclease 1; 3'-5' exonuclease ERI1; Eri-1 homolog; Histone mRNA 3'-end-specific exoribonuclease; Histone mRNA 3'-exonuclease 1; Protein 3'hExo; HEXO |
Target/Specificity | Recognizes endogenous levels of HEXO protein. |
Dilution | WB~~WB (1/500 - 1/1000) |
Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
Storage | Store at -20 °C.Stable for 12 months from date of receipt |
Name | ERI1 |
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Synonyms | 3'EXO, THEX1 |
Function | RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication (PubMed:14536070, PubMed:16912046, PubMed:17135487). A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates (PubMed:14536070, PubMed:16912046, PubMed:17135487). Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi) (PubMed:14961122). Required for binding the 5'-ACCCA-3' sequence present in stem-loop structure (PubMed:14536070, PubMed:16912046). Able to bind other mRNAs (PubMed:14536070, PubMed:16912046). Required for 5.8S rRNA 3'-end processing (By similarity). Also binds to 5.8s ribosomal RNA (By similarity). Binds with high affinity to the stem- loop structure of replication-dependent histone pre-mRNAs (PubMed:14536070, PubMed:17135487, PubMed:16912046). In vitro, does not have sequence specificity (PubMed:17135487). In vitro, has weak DNA exonuclease activity (PubMed:17135487). In vitro, shows biphasic kinetics such that there is rapid hydrolysis of the last three unpaired RNA nucleotides in the 39 flanking sequence followed by a much slower cleavage through the stem that occurs over a longer incubation period in the order of hours (PubMed:17135487). |
Cellular Location | Cytoplasm. Nucleus. Nucleus, nucleolus |
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Provided below are standard protocols that you may find useful for product applications.
Background
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HEXO. The exact sequence is proprietary.
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