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Anti-KCHIP1 Antibody

Rabbit polyclonal antibody to KCHIP1

     
  • WB - Anti-KCHIP1 Antibody AP61465
    Western blot analysis of KCHIP1 expression in CT26 (A), C6 (B), MCF7 (C), HEK293T (D), U87MG (E) whole cell lysates.
    detail
  • IH - Anti-KCHIP1 Antibody AP61465
    Immunohistochemical analysis of KCHIP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-KCHIP1 Antibody AP61465
    Immunofluorescent analysis of KCHIP1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession Q9NZI2
Other Accession Q9JJ57
Reactivity Human, Mouse, Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 26817 Da
Additional Information
Gene ID 30820
Other Names KCHIP1; VABP; Kv channel-interacting protein 1; KChIP1; A-type potassium channel modulatory protein 1; Potassium channel-interacting protein 1; Vesicle APC-binding protein
Target/Specificity Recognizes endogenous levels of KCHIP1 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
IH~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
IF~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name KCNIP1
Synonyms KCHIP1, VABP
Function Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents. Increases the presence of KCND2 at the cell surface.
Cellular Location Cell membrane; Peripheral membrane protein. Cytoplasm. Cell projection, dendrite {ECO:0000250|UniProtKB:Q8R426}
Tissue Location Isoform 1 and isoform 2 are expressed in brain and kidney. Isoform 1 is also expressed in liver, pancreas, skeletal muscle, small intestine and testis. Isoform 2 is also expressed in lung, pancreas, leukocytes, prostate and thymus
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human KCHIP1. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61465
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