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Anti-eIF4AI Antibody

Mouse monoclonal antibody to eIF4AI

     
  • WB - Anti-eIF4AI Antibody AP61578
    Western blot analysis of eIF4AI expression in 293T (A), Hela (B), HepG2 (C), mouse brain (D) whole cell lysates.
    detail
  • IH - Anti-eIF4AI Antibody AP61578
    Immunohistochemical analysis of eIF4AI staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-eIF4AI Antibody AP61578
    Immunofluorescent analysis of eIF4AI staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession P60842
Other Accession P60843
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW 46154 Da
Additional Information
Gene ID 1973
Other Names DDX2A; EIF4A; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1
Target/Specificity Recognizes endogenous levels of eIF4AI protein.
Dilution WB~~WB (1/1000 - 1/3000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)
IH~~WB (1/1000 - 1/3000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)
IF~~WB (1/1000 - 1/3000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name EIF4A1
Synonyms DDX2A, EIF4A
Function ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
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Background

KLH-conjugated synthetic peptide encompassing a sequence of human eIF4AI. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61578
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